International Journal of Engineering Technology and Scientific Innovation
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Jawonisi, O.I.; Olayemi, R.F.; Lawal, O.J.

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Department of Applied Science, College of Science & Technology, Kaduna Polytechnic, P.M.B 2021, Kaduna, Kaduna State, Nigeria

O.I., J., et al. "PHYSICOCHEMICAL AND PHYTOCHEMICAL EVALUATION OF Moringa oleifera POD AND SEED." IJETSI, vol. 2, no. 1, 2017, pp. 508-517,
O.I., J., R.F., O., & O.J., L. (2017). PHYSICOCHEMICAL AND PHYTOCHEMICAL EVALUATION OF Moringa oleifera POD AND SEED. IJETSI, 2(1), 508-517. Retrieved from
O.I., J., O. R.F., and L. O.J. "PHYSICOCHEMICAL AND PHYTOCHEMICAL EVALUATION OF Moringa oleifera POD AND SEED." IJETSI 2, no. 1 (2017), 508-517.

1.Brain RK, Turner DT.The practical evaluation of phytopharmaceuticals. Bristol: Wright-Scientechnica; 1975. 2. Dominguez XA. Metodos de investigacion fitoquimica. Mexico: Mexico, D. F; 1973.
3. Duke J. Handbook of Energy Crops: Moringa oleifera. New York: McGraw-Hill Medical; 1982.
4. Evan WC. Trease & Evans pharmacognosy. London: W.B Sanders; 2002.
5. Gaikwad SB, Mohan GK Rani MS. Phytochemicals for diabetes management. Pharma. Crops 2014; 5(1): 11-28.
6. Harbone JB. Phytochemical methods: a guide to modern techniques of plant analysis. London: Chapman & Hall; 1973.
7. Jayaprakasam B, Vareed SK, Olson LK, Nair MG. Insulin secretion by bioactive anthocyanins and anthocyanidins present in fruits. J. Agric. Food Chem. 2005; 53: 28-31.
8. Kar A. Pharmaceutical drug analysis. New Delhi: New Age International Limited; 2005.
9. Khandelwal KR. Practical pharmacognosy, techniques, and experiments. Pune: Nirali Prakashan; 2004.
10. Kokate CK, Purohit AP, Gokhale SB.Pharmacognosy. Pune, India: Nirali Prakashan; 2007.
11. Lucy D, Anoja S, Chu-Su Y. Alternative therapies for Type 2 diabetes. Alternative Med. Review.2002; 7, 45-58.
12. Mbaze LM, Poumale HM, Wansi JD, Lado JA, Khan SN, Iqbal MC, Ngadjui BT, Laatsch H. ?- Glucosidase inhibitory pentacyclic triterpenes from the stem bark of Fagara tessmannii (Rutaceae). J. Phytochem. 2007; 68(5): 591-595.
13. Narender, T., Khaliq, T., Singh, A.B., Joshi, M.D., Mishra, P., Chaturvedi, J.P., Srivastava, A.K., Maurya, R., & Agarwal, S.C. (2009).Synthesis of alpha-amyrin derivatives and their in vivo antihyperglycaemic activity. European J. Med. Chem. 2009; 44(3): 1215- 1222.
14. Perez GRM, Vargas SR. Triterpenes from Agarista Mexican as antidiabetic agents. Phytother. Res. 2002; 16: 55-58.
15. Ragasa CY, Tsai P, Shen CC. Terpenoids and Sterols from the Endemic and Endangered Philippine Trees, Ficus pseudopalma and Ficus ulmifolia.Philippine J. Sci. 2009; 138(2): 205-209.
16. Rao AV, Gurfinkel DM. The bioactivity of saponins: triterpenoid and steroidal glycosides. Drug Metabolism. Drug Interaction, 2000; 17, 211-235.
17. Ramachandran S, Prasad NR. Effect of ursolic acid, a triterpenoid antioxidant, on ultraviolet-B radiation- induced cytotoxicity, lipid peroxidation and DNA damage in human lymphocytes. Chem. Biol. Interaction, 2008; 176, 99-107.
18. Sofowora A. Medicinal Plants and Traditional Medicine in Africa. Ibadan: Spectrum Book Limited; 1993.
19. Trease CE, Evans WC. (1983). Pharmacognosy. London: Balliere Tindall Ltd; 1983.
20. Tsuda, T, Ueno Y, Yoshikawa T, Kojo H, Osawa T. Microarray profiling of gene expression in human adipocytes in response to anthocyanins. Biochem. Pharmacol. 2006; 7: 1184-1197.
21. Yasukawa K., Akihisa T, Oinuma H, Kasahara Y, Kimura Y, Yamanouchi S, Kamaki K, Takido M. Inhibitory effect of di- and trihydroxy triterpenes from the flowers of compositae on 12-otetradeaconylphorbol- 13- acetateinduced inflammation in mice. Biol. Pharm. Bull. 1996: 1329-1331.
22. Vessal M, Hemmati M, Vasei M. Hypoglycaemic effects of quercetin in streptozotocin-induced diabetic rats. Comp. Biochem. Physiol. Toxicol. Pharmacol. 2003; 13: 375- 364.
23. Waltner-Law ME, Wang XL, Law BK., Hall RK, Nawano M, Granner DK. Epigallocatechin gallate, a constituent of green tea, represses hepatic glucose production. J. Biol. Chem. 2002; 277: 3493334940.
24. WHO 2016.Global report on prevalence of diabetes Acessed December 30, 2016 from
25. Wild S, Roglic G, Green A, Singh W, Vaarala O. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes care, 2004; 27: 1047-1053.

Diabetes mellitus (DM) is a metabolic disorder without a known care hence the need for discovery of therapies for its treatment. The present study was under taken to investigate the physical properties and secondary metabolites in Moringa oleifera pod and seed with a view to identify the bioactive compounds with antidiabetic potentials in them. The extraction of Moringa oleifera pod and seed separately was carried out via maceration using 70% methanol. Fractionation of each macerate was done by liquid- liquid partitioning using solvents of varied polarity. Physicochemical properties and phytochemical screening were evaluated using standard laboratories procedures. The total ash, acid-insoluble ash, water-soluble ash, alcohol-soluble extractive value, water-soluble extractive value and moisture content were 6.5%, 3%,1%,3.2%4.2% and 5% for the pod and 3.0%,1%,0.5%,3.4%,4.2% and 5.3% respectively. The qualitative phytochemical screening revealed the presence of alkaloids, cardiac glycosides, flavonoids, glycosides, steroids, tannins and triterpenes in the 70% methanol extract and some of its fractions. Alkaloids, carbohydrates, cardiac glycosides, flavonoids, glycosides, saponins, steroids, tannins and triterpenes were present in the 70% methanol extract of the seed and some of its fractions. Anthraquinones (free and combined) and phlobatinnins were absent in the various extracts of the pod and seed. The result of the study shows that Moringa oleifera pod and seeds contain some of the phytochemicals with reported anti-diabetic properties. The presence of these bioactive compounds give credence to its use for management of diabetes mellitus in the rural areas in Nigeria. Moringa oleifera fruits can be explored for development of nutraceuticals with pure phytochemicals for management of diabetes mellitus.